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Please cite the following reference: Tuncbag N, Gursoy A, Keskin O. Identification of computational hotspots in protein interfaces: combining solvent accessibility and inter-residue potentials improves the accuracy. Bioinformatics. 2009 Jun 15;25(12):1513-20. [Link] Below you can try our prediction algorithm by entering the four letter PDB code of a protein or uploading your own structure file that is in the PDB format with the chain identifiers. Please do not submit PDB files containing only one chain. This will return an error! Hotpoint requires two chain identifiers which corresponds to a protein interface. Run our prediction algorithm for a particular input protein. Enter the four letter PDB code for automatic download from PDB: Or load your structure file from disk :        Enter the one letter chain identifiers for interface generation. Chain1: Chain2: Enter the distance threshold to fetch the interface residues. Default:The sum of van der Waals radii of two atoms + 0.5Å User defined distance threshold (Å):

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HotPOINT. : Hot Spot Prediction Server (v 1.00)  

For any problems or comments please send e-mail to Nurcan Tuncbag.