Below you can try our prediction algorithm by entering the four letter PDB code of a protein or uploading your own structure file that is in the PDB format with the chain identifiers. Please do not submit PDB files containing only one chain. This will return an error! Hotpoint requires two chain identifiers which corresponds to a protein interface. Tutorial

  • Please cite the following reference:
    Tuncbag N, Gursoy A, Keskin O. Identification of computational hotspots in protein interfaces: combining solvent accessibility and inter-residue potentials improves the accuracy. Bioinformatics. 2009 Jun 15;25(12):1513-20. [Link]

    Tuncbag N, Keksin O., Gursoy A., Hotpoint: hot spot prediction server for protein interfaces. Nucleic Acids Research, 2010, Vol. 38, Web Server issue